Rare large-tract development opportunity consisting of 37± acres of unimproved land located just off I-75 at Exit 404 in the fast-growing Alachua market. Zoned Business Highway, this site offers excellent flexibility for a wide range of commercial and mixed-use development concepts, supported by strong regional traffic counts and convenient interstate access. The property benefits from high visibility and accessibility due to its proximity to I-75, making it well-suited for highway-oriented commercial uses, destination retail, service-based businesses, hospitality, or employment-driven development. The current agricultural tax exemption allows for reduced holding costs during planning and entitlement phases. Situated near major demand drivers including the University of Florida, Shands Hospital, and popular eco-tourism attractions, the area continues to experience residential and commercial growth, increasing the site’s long-term value and development viability. With its substantial acreage, favorable zoning, and strategic location between Lake City and Gainesville near High Springs, this parcel provides a strong foundation for phased or master-planned development in one of North Central Florida’s expanding corridors. Buyer to confirm zoning, permitted uses, development density, and utility availability with local authorities. Potential Development Uses (Subject to Approval) Highway-oriented retail and service centers Hospitality (hotel, extended stay, or travel-related services) Office, medical, or flex space Mixed-use or employment-based development Commercial support uses aligned with regional growth and tourism. Listing ID: 09090-536276